The methods for isolation and identification of nanoparticles and for quantitative determination of nanoparticles in complex multicomponent mixed-phase systems are considered. These systems include most environmental objects, in particular, animal and plant organisms, agricultural raw materials, foodstuffs and various sorts of consumer goods. The published data and the results of authors' research concerning determination of various types of nanoparticles in biological samples by electron microscopy, chromatography and radioactive tracer method are generalized. The bibliography includes 161 references.