Abstract
We study the unzipping dynamics of individual DNA hairpins using nanopore force spectroscopy at different voltage ramp
rates and temperatures. At high ramp rates the critical unzipping voltage is proportional to log 
, where
is the voltage ramp. At low ramp values we observe a crossover to another regime with a weaker dependence on
. Here we report on the dependence of these two regimes on temperature. Remarkably, the unzipping kinetics can
be well described by a simple two-states model that predicts the existence of two asymptotic regimes: quasi-equilibrium
unzipping at low-voltage ramps and irreversible unzipping at high ramp rates.