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Determination of carbon-to-nitrogen ratio in the filamentous and heterocystous cyanobacterium Anabaena sp. PCC 7120 with single-cell soft X-ray imaging

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, , Citation T Teramoto et al 2017 J. Phys.: Conf. Ser. 849 012005 DOI 10.1088/1742-6596/849/1/012005

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tteramo@fc.ritsumei.ac.jp

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1 College of Science & Engineering, Ritsumeikan University, Shiga, 525-8577, JAPAN

2 SR center, Ritsumeikan University, Shiga, 525-8577, JAPAN

3 College of Life Sciences, Ritsumeikan University, Shiga, 525-8577, JAPAN

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1742-6596/849/1/012005

Abstract

Vegetative cells and heterocysts in the filamentous cyanobacterium Anabaena sp. PCC 7120 were observed by soft X-ray microscopy. Carbon-to-nitrogen (C/N) ratio of each cell was estimated by the difference of the absorbance of the images below and above the nitrogen K-edge absorption. It was revealed that the C/N ratios in vegetative cells and heterocysts are 4.54 and 2.46, respectively.

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10.1088/1742-6596/849/1/012005